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KMID : 0624620120450050293
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BMB Reports
2012 Volume.45 No. 5 p.293 ~ p.298
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Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana
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Zhang Liang
Meng Fei
Li Yuzhen
Kang Mingjiang
Guo Xingqi
Xu Baohua
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Abstract
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The p38 mitogen-activated protein kinase (MAPK) is involved in various processes, including stress responses, development, and differentiation. However, little information on p38 MAPK in insects is available. In this study, a p38 MAPK gene, Accp38b, was isolated from Apis cerana cerana and characterized. The quantitative real-time PCR (Q-PCR) analysis revealed that Accp38b was induced by multiple stressors. Notably, the expression of Accp38b was relatively higher in the pupae phase than in other developmental phases. During the pupae phase, Accp38b expression was higher in the thorax than in the head and abdomen and higher in the fat body than in the muscle and midgut. Immunohistochemisty showed significant positive staining of Accp38b in sections from the brain, eyes, fat body, and midgut of A. cerana cerana. These results suggest that Accp38b may play a crucial role in stress responses and have multiple aspects function during development.
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KEYWORD
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Apis cerana cerana, Expression analysis, Immunohistochemistry, Stress response, p38 MAPK
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